Different Stages Of Development Research Articles

Micro-CT and histological examination of accessory canals in 34 equine cheek teeth

Accessory canals and apical deltas have been extensively studied in human dentistry. Their clinical role as a difficult to clean reservoir for bacteria during endodontic treatments has been well described. Many papers describe in detail the pulp anatomy of equine dentition but little attention has been given to their apical ramifications. The goal of this paper is to describe the presence and anatomy of these accessory canals and apical deltas in healthy equine cheek teeth and discuss their possible relevance in the light of equine endodontics. To accomplish this, 15 maxillary and 19 mandibular healthy cheek teeth were collected ranging from Triadan 06 s to 11 s with eruption ages from 4 to 9 years. Their root anatomy was documented in detail based on micro computed tomography images. A subset of 3 teeth also underwent histological examination. Accessory canals were found in all but two teeth examined. Up to 44 accessory canals per tooth have been found with locations ranging from the root furcation down to the apex of the root and with highly variable diameters. Apical deltas in different stages of development were found in 84% of the roots. The presence of accessory canals identified on microCT images could be confirmed using histological examination although some of them were obliterated by reparative dentin. Accessory canals can be found in most equine cheek teeth and add complexity to their endodontic anatomy. This could have important implications for their treatment in case of pulp pathology. In humans, failure to remove bacterial biofilm from such canals has been associated with failure of endodontic treatment. Research on diseased equine teeth is required to gain a better understanding of their clinical relevance in horses.

The Public Role of Italian Sociology: Among Institutions, Universities and Social Engagement

AbstractSociology has played an important role in Italy, although its centrality in public debate has fluctuated, and it has been supported or opposed by political power at different historical moments. Contemporary Italian sociology has developed since the 1950s and has been influenced in various ways by European and American sociological theories. Sociology has played an important role in Italian public debate and has offered a critical and scientific perspective on the country’s social reality. However, this role should be understood in the context of the different stages of development that have accompanied the academic growth of the discipline and the presence of sociologists in ‘policy making’ processes. In the latter sense, Italian sociology has played an important role in the formation of public policies, as it offers a scientific analysis of social problems and proposes solutions based on empirical research. Within this framework, the training processes also need to be situated within what has been defined—albeit with conceptual ambiguity—as ‘professional sociology’, which involves important figures such as social workers, psychologists, and health workers. However, sociology has also played an important political role both in supporting the actions of policy makers and in analysing the contradictions of the capitalist system. This article also analyses the emergence of a ‘transformative’ positional sociology alongside the traditional public sociology. The goal of this transformative approach is to counteract the public role of Italian sociology, which seems to have lost its critical-explanatory vocation without ever having truly acquired a propositional function in the social context.

The spatial and social correlates of neighborhood morphology: Evidence from building footprints in five U.S. metropolitan areas.

Recent advances in quantitative tools for examining urban morphology enable the development of morphometrics that can characterize the size, shape, and placement of buildings; the relationships between them; and their association with broader patterns of development. Although these methods have the potential to provide substantial insight into the ways in which neighborhood morphology shapes the socioeconomic and demographic characteristics of neighborhoods and communities, this question is largely unexplored. Using building footprints in five of the ten largest U.S. metropolitan areas (Atlanta, Boston, Chicago, Houston, and Los Angeles) and the open-source R package, foot, we examine how neighborhood morphology differs across U.S. metropolitan areas and across the urban-exurban landscape. Principal components analysis, unsupervised classification (K-means), and Ordinary Least Squares regression analysis are used to develop a morphological typology of neighborhoods and to examine its association with the spatial, socioeconomic, and demographic characteristics of census tracts. Our findings illustrate substantial variation in the morphology of neighborhoods, both across the five metropolitan areas as well as between central cities, suburbs, and the urban fringe within each metropolitan area. We identify five different types of neighborhoods indicative of different stages of development and distributed unevenly across the urban landscape: these include low-density neighborhoods on the urban fringe; mixed use and high-density residential areas in central cities; and uniform residential neighborhoods in suburban cities. Results from regression analysis illustrate that the prevalence of each of these forms is closely associated with variation in socioeconomic and demographic characteristics such as population density, the prevalence of multifamily housing, and income, race/ethnicity, homeownership, and commuting by car. We conclude by discussing the implications of our findings and suggesting avenues for future research on neighborhood morphology, including ways that it might provide insight into issues such as zoning and land use, housing policy, and residential segregation.

Carbon dioxide enrichment affected flower numbers transiently and increased successful post-pollination development stably but without altering final acorn production in mature pedunculate oak (Quercus robur L.)

Acorn production in oak (Quercus spp.) shows considerable inter-annual variation, known as masting, which provides a natural defence against seed predators but a highly-variable supply of acorns for uses such as in commercial tree planting each year. Anthropogenic emissions of greenhouse gases have been very widely reported to influence plant growth and seed or fruit size and quantity via the ‘fertilisation effect’ that leads to enhanced photosynthesis. To examine if acorn production in mature woodland communities will be affected by further increase in CO2, the contents of litter traps from a Free Air Carbon Enrichment (FACE) experiment in deciduous woodland in central England were analysed for numbers of flowers and acorns of pedunculate oak (Quercus robur L.) at different stages of development and their predation levels under ambient and elevated CO2 concentrations. Inter-annual variation in acorn numbers was considerable and cyclical between 2015 and 2021, with the greatest numbers of mature acorns in 2015, 2017 and 2020 but almost none in 2018. The numbers of flowers, enlarged cups, immature acorns, empty acorn cups, and galls in the litter traps also varied amongst years; comparatively high numbers of enlarged cups were recorded in 2018, suggesting Q. robur at this site is a fruit maturation masting species (i.e., the extent of abortion of pollinated flowers during acorn development affects mature acorn numbers greatly). Raising the atmospheric CO2 concentration by 150 μL L−1, from early 2017, increased the numbers of immature acorns, and all acorn evidence (empty cups + immature acorns + mature acorns) detected in the litter traps compared to ambient controls by 2021, but did not consistently affect the numbers of flowers, enlarged cups, empty cups, or mature acorns. The number of flowers in the elevated CO2 plots’ litter traps was greater in 2018 than 2017, one year after CO2 enrichment began, whereas numbers declined in ambient plots. Enrichment with CO2 also increased the number of oak knopper galls (Andricus quercuscalicis Burgsdorf). We conclude that elevated CO2 increased the occurrence of acorns developing from flowers, but the putative benefit to mature acorn numbers may have been hidden by excessive pre- and/or post-dispersal predation. There was no evidence that elevated CO2 altered masting behaviour.

Depression, stress, and anxiety versus internet addiction in early and middle adolescent groups: the mediating roles of family and school environments

BackgroundFamily and school environment play a crucial role across the different developmental stages of adolescence. This paper investigates the potential mediating role of family and school environments in the relationship between the three psychosocial predictors of depression, anxiety, stress, and Internet addiction (IA). Specifically, it focuses on the two stages of early and middle adolescence.MethodsThe study involved a survey of 407 adolescents from Qatar, comprising 250 early adolescents and 157 middle adolescents. Inclusion criteria for the study included adolescents between the ages of 10 to 17 years old, residents of Qatar and studying in a Qatar-based school. To assess the constructs of the three psychosocial predictors, IA, family environment, the study utilized the Depression, Stress, and Anxiety Scale (DASS), the Internet Addiction Diagnostic Questionnaire (IADQ), and the Brief Family Relationship Scale, respectively. School environment was measured using questions from the “Health Behavior in School-aged Children: WHO Collaborative Cross-National survey/study (HBSC) 2013–2014. The study applied standard mediation analysis between the DASS components and IA with family and school environment as the mediators.ResultsResults from the mediation analysis reveal insights into the relationships between psychosocial predictors and IA. The findings indicate that family and school environments partially mediated the relationship with regards to depression, stress, and anxiety in early adolescents. In middle adolescents, family environment partially mediated the relationship with depression and stress and fully mediating the relationship with anxiety. Meanwhile, school environment only exhibited partial mediation in the relationship with anxiety in middle adolescence.ConclusionsThese results highlight the crucial role parents and schools play in addressing problematic technology usage that develops as a response to depression, anxiety, and stress among adolescents. Moreover, the study reveals nuances in the mediating role of family and school environment in early and middle adolescence. This highlights the evolving nature of these influences across the different stages of development. Notably, this study contributes to the literature by moving beyond the conventional focus on the so-called WEIRD population, and offering valuable insights from a region that is underrepresented in current research.

Caste Differences in Child Growth: Disentangling Endowment and Investment Effects

Using the fourth round of the Indian National Family Health Survey (NFHS-4), and subsequently replicating our results using the fifth round (NFHS-5), we document differential child physical growth patterns across caste groups in India, demonstrating that lower caste children are born shorter and grow less quickly than children from higher-caste households. We then show that, in line with work from previous rounds of the NFHS, these differences are largely explainable by observable covariates, particularly maternal characteristics and household wealth variables. Our research also reveals a previously undocumented dynamic, that the influence of these variables changes as children develop, and suggests that caste-gaps are the result of multiple mechanisms impacting the child growth process at different stages of development. Using age-disaggregated decomposition methods, we demonstrate that health endowment related variables (e.g. maternal height) largely explain birth length gaps, and that variables related to health investments (e.g. household wealth, health care usage) become increasingly influential as children age. Children from lower caste households thus face two margins generating height gaps as they age: a persistent endowment disparity present from birth, and a post birth investment differential that exacerbates the initial deficit.

Short communication: Characterizing arterial and venous blood gases over the gas exchange surface, the chorioallantoic membrane, of embryonic American alligators (Alligator mississippiensis) at two points of development

Assessments of arterial and venous blood gases are required to understand the function of respiratory organs in animals at different stages of development. We measured blood gases in the arteries entering and veins leaving the chorioallantoic membrane (CAM) in embryonic alligators (Alligator mississippiensis). The CAM accounts for virtually all gas exchange in these animals, and we hypothesized that the CAM vasculature would be larger in eggs incubated in hypoxia (10% O2 for 50% or 70% of incubation), which would be reflected in a lower partial pressure of CO2 (PCO2). Contrary to this hypothesis, our measurements revealed no effects of hypoxic incubation on PCO2, and seemingly no increase in vascularization of the CAM in response to incubation in 10% O2. PCO2 was lower on the venous side, but only significantly different from arterial blood at 70% of incubation. The calculated blood flow to the CAM increased with development and was lower in both groups of alligators that had been incubated in hypoxia. Future studies should include measurements of blood parameters taken from embryos held in conditions that mirror incubation O2 levels, in combination with direct measurements of CAM artery blood flow.

[Artículo traducido] Variación de las propiedades mecánicas del hueso inmaduro en las etapas más tempranas del desarrollo

Introduction Bone as a material varies its composition and mechanical properties throughout life. Although these variations are better understood in adulthood, there is little experimental information on the variation of these properties in early stages of development. The objective of this study is to analyze the mechanical behavior and chemical properties of cortical bone tissue from two animal species in these earliest stages.Material and methodology Twenty specimens of cortical bone were manufactured from bovine and ovine species that were in different stages of development (feeding exclusively on breast milk, in the transition period to feed or pasture, and young animals but on a solid food diet). The specimens were subjected to tensile tests, recorded with a high-speed camera to obtain deformation maps. Measurements of the tensile force until the specimen broke were also carried out. A fractographic study was carried out with a scanning electron microscope to analyze the fracture surface and an analysis of the amount of calcium in each of the specimens using X-ray dispersion spectroscopy.Results A statistically significant and positive correlation was found between the elastic modulus of the specimens and their calcium content. A trend towards more rigid behavior with age was observed.Conclusions Young bone tissue tends to stiffen with age as the calcium content increases with an increase in elastic modulus.

RESEARCH ON THE DRIVING FORCE OF CHINA'S RURAL TOURISM DEVELOPMENT BASED ON VILLAGE LANDSCAPE RESOURCES

This paper mainly adopts qualitative research, summarizes the development stage of rural tourism through in-depth interviews and second-hand data analysis, and uses the root theory to study the driving force of rural tourism development based on village landscape resources. From the different stages of rural tourism development, the driving force of different stages of development is proposed, the role of attraction, endogenous force, development force, and subsequent power of rural tourism development on rural tourism development is explored, and the model diagram of the driving force of rural tourism development is constructed.

К изучению биологии судака (Sander lucioperca) в изменившихся экологических условиях северной части Аграханского залива Каспийского моря

The northern part of the Agrakhan Bay, which used to play a peculiar role of a receiving reservoir for migratory and semi-migratory fish migrating to spawn and wintering, and served as a kind of nursery for the development of larvae of their young, rolling here from spawning-growing reservoirs and the lower reaches of the Terek, in recent years, as a result of a critical decrease in river flow and a drop in the level of the Caspian. It has ceased to perform its main functions to increase fish stocks in the Terek-Caspian fisheries area. Among semi-migratory fish, the walleye is of particularly important fishery importance, as the largest and most valuable representative of the Percidae family in commercial and food relations. It was found that the structure of the walleye population in the northern part of the Agrakhan Bay in the last 2018-2021 turned out to be the most susceptible to unaccounted withdrawal, and its number is in a depressed state. It was revealed that the current level of reproduction of walleye in the bay turned out to be relatively lower as a result of intensive overgrowth and weak water availability. Histological examination of the gonads showed that the ovaries of walleye in the IV stage of maturity are for five months, from the end of October to April. It is established that the growth rate at different stages of development depends on environmental factors (hydro-hydrochemical and temperature regime of reservoirs, regime, salt balance, etc.) as well as their food supply.

Evaluation of fruit detachment forces and related characteristics reveals differential fruit detachments at developmental stages and cultivar differences in cashew

Cashew is an edible tree nut with great nutritional and industrial importance. Cashew fruit is unique because the true fruit (nut) grows attached to a swollen flower pedicel known as a hypocarp or cashew apple. The fruit detachment force (FDF), a quantitative measurement of the detachment of fruits from the stalk, is considered as an important parameter for the evaluation of the harvesting efficiency of machines, and the cultivar's potential for machine harvesting, and tolerances to biotic and abiotic stresses. There have been no studies on the evaluation of FDFs in cashew to date. In the current study, we investigated the detachment forces of fruits at ten different stages of development according to the BBCH (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie) scale, a uniform coding system for different growth or phenological stages (701, 703, 705, 707, 709, 811, 813, 815, 817, 819) in ten cashew cultivars. Fruit weight (FW), FDF/FW ratio, and fruit firmness (FF) for different fruit development stages and fruit stalk scar area (SSA) of matured (819 stage) fruits were also studied. FDF, FW, FDW/FW and FF varied significantly at the different stages of fruit development among the studied cultivars. The FDF increased from 701 to 709 stages and then decreased from 709 to 819 stages in all the studied cultivars. The FDW/FW ratio reduced with the development and maturity of the fruit. In matured fruits (811–819 stages), FW and FF showed significant negative (p value = 0.028) and positive (p value = 0.015.) correlations, respectively, with the FDF. In contrast, the SSA had no significant influence on the FDFs. As the FDF has direct implications on the fruit removal efficiency, the results of this study will be useful for designing harvesting machines, screening genotypes for stress tolerance, and identifying the optimal harvesting stage in cashew.

More Inclusive and Wider Sources: A Comparative Analysis of Data and Political Journalists on Twitter (Now X) in Germany

Women are underrepresented in many areas of journalistic newsrooms. In this paper, we examine if this established effect persists in the new forms of journalistic communication, namely social media networks. We use mentions, retweets, and hashtags as measures of journalistic amplification and legitimation. Furthermore, we compare two groups of journalists in different stages of development: political and data journalists in Germany in 2021. Our results show that journalists identified as women tend to favor other women journalists in mentions and retweets on Twitter (now called X), compared to men. While both professions are dominated by men, with a high share of tweets authored by men, women mention and retweet other women more than their male colleagues. Female data journalists also leverage different sources than men. In addition, we found data journalists to be more inclusive of non-member sources in their networks compared to political journalists.

Polycentric Urban Spatial Structure Identification Based on Morphological and Functional Dimensions: Evidence from Three Chinese Cities

Regarding whether a polycentric urban spatial structure is a sustainable urban development model, various studies use different employment center identification methods, resulting in inconsistent conclusions. This paper takes Hangzhou, Wuhan, and Nanning, three Chinese cities at different stages of development as cases, and adopts various methods to identify employment centers to compares the polycentric spatial structure differences from morphological and functional dimensions, and puts forward a multi-dimensional perspective for analyzing the characteristics of the polycentric spatial structure. The results show that the polycentric model of GL_SC threshold method has the relatively good explanatory power, which can best represent the characteristics of urban development in China. The explanatory power of the polycentric model of the Wuhan commuter flow method (adjusted R-squared value is 0.37) is slightly weaker than that of the GL_SC threshold method (adjusted R-squared value is 0.40), indicating that the agglomeration development forces are equivalent to the decentralized development forces and it is a balanced polycentric development structure. In Hangzhou and Nanning, the GL_SC threshold method (adjusted R-squared values are 0.28 and 0.36, respectively) is stronger than the commuter flow method (adjusted R-squared values are 0.19 and 0.33, respectively), which reflects the strong characteristics of agglomeration polycentric development. However, from the comparison of the number and distribution of employment center identification, the polycentric development characteristics of Hangzhou are stronger than those of Nanning. The comparison of the identification results of the morphological and functional dimensions shows that the two dimensions as a unified evaluation process can more fully reflect the characteristics of an urban polycentric development structure, thereby providing preliminary research support for the study of the impact of polycentric structure on economic development, air pollution, work and residential commuting, etc.

The impact of rivers and lakes on urban transportation expansion: A case study of the century-long evolution of the road network in Wuhan, China.

Throughout history, rivers and lakes have wielded a profound influence on the dynamics of urban transportation expansion. To illustrate this phenomenon, we turn to the century-long evolution of the road network in Wuhan, China, as a case study. The study aims to explore the relationship framework between water bodies and urban transportation, characterized by the sequence of "strong connection" to "weakened connection", then to "mutual restriction", and ultimately to "mutual benefit". Additionally, the analysis of the impact mechanisms of rivers and lakes on urban transportation at different stages of development is also a key research objective. To facilitate our exploration, we select the road networks in Wuhan from four years of 1922, 1969, 1995, and 2023 as the primary research subjects. By establishing water buffers, we scrutinize the evolving characteristics of riverside and lakeside transportation amidst the city's expansion. Based on the modified shortest path model, we introduce the innovative concepts of "Detour Index" and "Weighted Detour Index" to assess the road accessibility of each node in the city based on its inherent environmental conditions. This allows for the effective analysis of the potential impact of water bodies as "obstacles" on the road network at different stages of urban development. The study found that in the areas adjacent to the rivers and lakes in Wuhan, there is insufficient road accessibility based on their inherent environmental conditions. Particularly, some areas along the rivers may become "terminals" in the urban road network. Furthermore, during the process of urban expansion, the correlation between the urban road network and rivers continues to weaken, while the correlation with lakes continues to strengthen. These conclusions can provide valuable insights for the planning of urban roads near water bodies.

PIEZO1 acts as a cancer suppressor by regulating the ROS/Wnt/β-catenin axis.

PIEZO1 works differently in different cancers and at different stages of development. The objective of the current study was to explore the function and underlying mechanism of PIEZO1 in lung adenocarcinoma (LUAD) cells. Different LUAD cell lines were treated with PIEZO1 inhibitor (GsMTx4) and agonist (Yoda1), and the expression of PIEZO1 in LUAD cells was detected using real-time quantitative PCR (RT-qPCR) and western blotting. The effects of PIEZO1 on invasion, migration and epithelial-mesenchymal transition (EMT) markers protein expression of LUAD cells were detected using the MTT assay, flow cytometry, transwell assay, wound-healing assay, and western blotting. Reactive oxygen species (ROS) agonists (BAY 87-2243) and inhibitors (NAC) and Wnt/β-catenin pathway inhibitors (iCRT3) were selected to treat A549 cells to investigate the mechanism of PIEZO1 on ROS production and Wnt/β-catenin expression in A549 cells. In A549, NCI-H1395, and NCI-H1975 cells, GsMTx4 promoted cell proliferation, invasion, migration, upregulated EMT-related marker protein expression, and inhibited cell apoptosis, while Yoda1 exerted effects opposite to those of GsMTx4. In A549 cells, GsMTx4 can reduce ROS production, it also inhibited ROS production, apoptosis, and downregulated proapoptotic markers induced by BAY 87-2243. Importantly, BAY 87-2243 blocked the effect of GSMTX4-induced Wnt/β-catenin overexpression. Similarly, Yoda1 can reduce the effect of NAC. In addition, iCRT3 can block the upregulation of EMT-related marker proteins by GsMTx4, and increase apoptosis and decrease cell invasion and migration. In summary, PIEZO1 acts as a cancer suppressor by regulating the ROS/Wnt/β-catenin axis, providing a new perspective on the role of mechanosensitive channel proteins in cancer.

What do private providers of home care want? An analytical framework

ABSTRACT Private for-profit home care providers are key participants in European long-term care regimes. Yet, their policy preferences toward market making and market development remain under-researched. We present an analytical framework that identifies and explains those preferences. It outlines three key types of policies that shape home care markets: regulatory, financial and labour policies. We illustrate private providers’ views on those policies by reference to Ireland’s home care regime. Irish home care is a compelling case for this study. A major package of regulatory reforms is currently being introduced and private providers have outlined clear preferences about it. Our paper is based on interviews with 14 leading individuals involved in the home care sector. We find and explain how private providers wish to increase regulation over their activities; to split purchaser and provider roles; to reduce private forms of financing; and to expand the pool of labour available to them. We discuss how those preferences shed light on the political economic power dynamics that shape the Irish care regime. Our analytical framework may be applicable to other welfare markets at different stages of development and in other institutional contexts.

Histological, immunohistochemical and serological investigations of the ovary during follicular phase of estrous cycle in Saidi sheep

BackgroundSaidi sheep are the most abundant ruminant livestock species in Upper Egypt, especially in the Assiut governorate. Sheep are one of the most abundant animals raised for food in Egypt. They can convert low-quality roughages into meat and milk in addition to producing fiber and hides therefore; great opportunity exists to enhance their reproduction. Saidi breed is poorly known in terms of reproduction. So this work was done to give more information on some hormonal, oxidative, and blood metabolites parameters in addition to histological, histochemical and immunohistochemical investigations of the ovary during follicular phase of estrous cycle. The present study was conducted on 25 healthy Saidi ewes for serum analysis and 10 healthy ewes for histological assessment aged 2 to 5 years and weighted (38.5 ± 2.03 kg).ResultsThe follicular phase of estrous cycle in Saidi sheep was characterized by the presence of ovarian follicles in different stages of development and atresia in addition to regressed corpus luteum. Interestingly, apoptosis and tissue oxidative markers play a crucial role in follicular and corpus luteum regression. The most prominent features of the follicular phase were the presence of mature antral (Graafian) and preovulatory follicles as well as increased level of some blood metabolites and oxidative markers. Here we give a new schematic sequence of ovarian follicles in Saidi sheep and describing the features of different types. We also clarified that these histological pictures of the ovary was influenced by hormonal, oxidative and blood metabolites factors that characterizes the follicular phase of estrous cycle in Saidi sheep.ConclusionThis work helps to understanding the reproduction in Saidi sheep which assist in improving the reproductive outcome of this breed of sheep. These findings are increasingly important for implementation of a genetic improvement program and utilizing the advanced reproductive techniques as estrous synchronization, artificial insemination and embryo transfer.

Experimental biology can inform our understanding of food insecurity.

Food insecurity is a major public health issue. Millions of households worldwide have intermittent and unpredictable access to food and this experience is associated with greater risk for a host of negative health outcomes. While food insecurity is a contemporary concern, we can understand its effects better if we acknowledge that there are ancient biological programs that evolved to respond to the experience of food scarcity and uncertainty, and they may be particularly sensitive to food insecurity during development. Support for this conjecture comes from common findings in several recent animal studies that have modeled insecurity by manipulating predictability of food access in various ways. Using different experimental paradigms in different species, these studies have shown that experience of insecure access to food can lead to changes in weight, motivation and cognition. Some of these studies account for changes in weight through changes in metabolism, while others observe increases in feeding and motivation to work for food. It has been proposed that weight gain is an adaptive response to the experience of food insecurity as 'insurance' in an uncertain future, while changes in motivation and cognition may reflect strategic adjustments in foraging behavior. Animal studies also offer the opportunity to make in-depth controlled studies of mechanisms and behavior. So far, there is evidence that the experience of food insecurity can impact metabolic efficiency, reproductive capacity and dopamine neuron synapses. Further work on behavior, the central and peripheral nervous system, the gut and liver, along with variation in age of exposure, will be needed to better understand the full body impacts of food insecurity at different stages of development.

Toward mechanistic medical digital twins: some use cases in immunology.

A fundamental challenge for personalized medicine is to capture enough of the complexity of an individual patient to determine an optimal way to keep them healthy or restore their health. This will require personalized computational models of sufficient resolution and with enough mechanistic information to provide actionable information to the clinician. Such personalized models are increasingly referred to as medical digital twins. Digital twin technology for health applications is still in its infancy, and extensive research and development is required. This article focuses on several projects in different stages of development that can lead to specific-and practical-medical digital twins or digital twin modeling platforms. It emerged from a two-day forum on problems related to medical digital twins, particularly those involving an immune system component. Open access video recordings of the forum discussions are available.

Vegetation indices monitoring by using copernicus data in the old-growth forests of the Republic of Srpska/Bosnia and Herzegovina

IntroductionOld-growth forests (OGFs) are vital for global biodiversity, acting as irreplaceable carbon sinks, fostering ecosystem stability, and preserving unique ecological and cultural heritage. There are three old-growth forests in the Republic of Srpska/Bosnia and Herzegovina (Lom, Janj, and Perućica). They are dominated by mixed forests of beech, fir, and spruce (Piceo-Abieti-Fagetim illyricum). The satellite imagery results confirm that the vegetational indices are crucial for monitoring old-growth forests. Vegetation indices provide significant information for monitoring forest ecosystems. Based on Copernicus Sentinel-2 images, remote sensing is enabled, providing data in various spatial and spectral resolutions. The analysis of images related to old-growth forests allows for monitoring the spatial dynamics of changes in vitality and the health status of forests. This research aims to characterize old-growth forests from space through vegetation indices to obtain a more in-depth analysis of the ecological condition of these forests.MethodsThe research methodology includes the application of Sentinel 2A satellite imagery, which is taken from the European Space Agency (ESA) database. The Sentinel 2 mission consists of two identical satellites (Sentinel 2A and Sentinel 2B) that have multispectral sensors with 13 spectral channels (bands) at spatial resolutions of 10, 20, and 60 m. The analysis included the normalized difference vegetation index (NDVI), enhanced vegetation index (EVI), soil-adjusted vegetation index (SAVI), and visible atmospheric resistance index (VARI). For determining the significance of differences in vegetation index magnitudes between old-growth forests, a one-way analysis of variance with equal repetitions was employed.Results and discussionThe parts of old-growth forests are located in different stages of development, and the trees in them are characterized by different degrees of vitality and have different values of vegetation indices. It is assumed that parts of old-growth forests in the terminal phase of development with a large number of diseased and rotting trees have the smallest sizes of vegetation indices. This research aims to characterize old-growth forests from space through vegetation indices to obtain a more in-depth analysis of the ecological condition of these forests. Using satellite imagery and their analysis of the obtained sizes of vegetation indices, it is observed that there is a significant difference in their sizes between old-growth forests. The Janj old-growth forest stands out as the area with the highest NDVI values, followed by the Lom old-growth forest, while the Perućica old-growth forest has the lowest NDVI values. However, the Perućica old-growth forest has significantly higher values of EVI and SAVI vegetation indices, while the other two old-growth forests have approximately similar values. The VARI index values are highest in the Lom old-growth forest, slightly lower in the Perućica old-growth forest, and lowest in the Janj old-growth forest. The results of these studies indicate statistically significant differences in vegetation index magnitudes between old-growth forests. The application of Copernicus satellite data enables the monitoring of changes in vegetation index values, which is of great assistance in spatially defining the developmental stages in old-growth forests (OGFs). Considering the relatively small number of sample areas covered by these studies, similar research needs to be conducted on a larger number of sample areas (replications), especially during the vegetation period.